落尘之木博客
TO BE THE BEST!

生物PCR测序引物以及杂交探针Primer Premier v6.0

Primer Premier是一款由加拿大的Premier公司开发的专业用于PCR或测序引物以及杂交探针的设计,评估的软件,其主要界面是分为序列编辑窗口(Genetank),引物设计窗口(Primer Design),酶切分析窗口(Restriction Sites)和纹基分析窗口(Motif)。

Primer Premier软件的主要功能分四种,即引物设计、限制性内切酶位点分析、DNA 基元(motif)查找和同源性分析功能。前三种为其主要功能。此外,该软件还有一些特殊功能,其中最重要的是设计简并引物,另外还有序列”朗读”、DNA 与蛋白序列的互换、语音提示键盘输入等等。

Primer Premier软件还可以针对模板DNA 的来源以相应的遗传密码规则转换DNA 和氨基酸序列。软件共给出八种生物亚结构的不同遗传密码规则供用户选择,有纤毛虫大核(Ciliate Macronuclear)、无脊椎动物线粒体(Invertebrate Mitochondrion)、支原体(Mycoplasma)、植物线粒体(Plant Mitochondrion)、原生动物线粒体(Protozoan Mitochondrion)、一般标准(Standard)、脊椎动物线粒体(Vertebrate Mito-chondrion)和酵母线粒体(Yeast Mitochondrion)。

Primer Premier软件有优秀的引物自动搜索功能,同时可进行部分指标的分析,而且容易使用,已被引物设计行业人士认为是一款相当不错的软件。

A Comprehensive PCR Primer Design Software

Primer Premier’s search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in a ranked order. Load the gene of interest from NCBI, select a search range, sit back and let Primer Premier pick the best possible primers for you.

Primer Design for Standard PCR Assays

Primer Premier is the most comprehensive software to design and analyze PCR primers.

Primer Premier’s search algorithm finds optimal PCR, multiplex and SNP genotyping primers with the most accurate melting temperature using the nearest neighbor thermodynamic algorithm. Primers are screened for secondary structures, dimers, hairpins, homologies and physical properties before reporting the best ones for your sequence, in ranked order. Equipped with a handy calculator, you can easily manipulate sequences and analyze the results of your primer design.

Primer Design for SNP Genotyping Assays

With Primer Premier, you can load sequences from dbSNP and have the primers designed flanking the SNP selected. Hundreds of unpublished SNPs can also be loaded by specifying them as variation features in standard GenBank/dbSNP files. After specifying the SNPs, primers can be designed to amplify them for detection using a probe-based chemistry.

官方网站:http://www.premierbiosoft.com/primerdesign/index.html

下载地址
城通网盘:
https://luochenzhimu.ctfile.com
百度网盘:
https://pan.baidu.com/s/1o90hbS2
盛天云盘:
https://pan.stnts.com/s/0PzENqa
蓝奏云:
https://pan.lanzou.com/i0digna

安装指引:
运行安装Primer Premier 6.0 Installer.exe安装
替换C:\Program Files\Primer Premier 6.0\http\Dwww.premierbiosoft.com\P80\DMIUpdater\DMPP\DM600目录下的RMToolkit.jar
如果更改了安装目录可以按照更改的路径找到RMToolkit.jar替换

Primer Premier 6.24试用版下载:
Win64:
http://www.premierbiosoft.com/DATAFILES/PP624InstallerWin64.exe
Mac:
http://www.premierbiosoft.com/DATAFILES/PP624Installer.dmg

您的支持将鼓励我们继续创作!

[微信] 扫描二维码赞赏

[支付宝] 扫描二维码赞赏

正在跳转到PayPal...
赞(0)
转载请注明落尘之木博客 » 生物PCR测序引物以及杂交探针Primer Premier v6.0

评论 1

评论前必须登录!

 

  1. #1
    Google Chrome 63 Google Chrome 63 Windows 10 Windows 10

    博主也是学生物的?

    lind6个月前 (01-16)